Degradative processing of internalized insulin in isolated adipocytes.
نویسندگان
چکیده
منابع مشابه
Internalization and molecular processing of insulin receptors in isolated rat adipocytes.
The cellular fate of insulin receptors in isolated rat adipocytes was studied by using a biologically active photosensitive insulin derivative, B2(2-nitro-4-azidophenylacetyl)-des-PheB1-insulin (NAPA-DP-insulin), to photoaffinity label the insulin receptors. Insulin receptors specifically labeled with 125I-labeled NAPA-DP-insulin were identified by NaDodSO4/polyacrylamide gel electrophoresis an...
متن کاملIsolated Rat Adipocytes RELATIONSHIPS BETWEEN INSULIN RECEPTORS AND INSULIN ACTION
was plotted as a function of the percent instlin effect on uptake, control cells and cells from 24-hfasted rats gave comparable results, while cells from 48and 72-h-fasted animals still took Up less hexose for any amount of bound insulin. The effects of fasting on 3-0-methyl glucose uptake were comparable to the 2-deoxy-gltucose data. In conclusion: (a) insulin binding is increased durinig fast...
متن کاملKinetics of insulin receptor internalization and recycling in adipocytes. Shunting of receptors to a degradative pathway by inhibitors of recycling.
The kinetics of receptor internalization and recycling was directly determined in adipocytes by measuring 125I-insulin binding to total, intracellular, and cell-surface insulin receptors. In the absence of insulin 90% of all receptors were on the cell-surface and 10% were intracellular. Insulin (100 ng/ml) rapidly altered this distribution by translocating surface receptors to the cell-interior...
متن کاملCinnamon Extract Changes the Expression of miRNAs26-b, 29a, 223 and320 in Insulin Resistant Adipocytes
Objective: Insulin resistance (IR) is the major cause in Type 2 diabetes mellitus (T2DM). Expression of some miRNAs can be changed in response to a drug treatment for IR, and used as the biomarker in IR. This study set out to determine the effect of cinnamon extract (cinnamaldehyde) on some miRNAs expression in IR adipocytes. Materials and Methods: In this In-vitro study the 3T3L1 cells were e...
متن کاملMetformin and Pioglitazone Reduce Gene Expression of Inflammatory Factors in Insulin Resistant and Hypertrophied Adipocytes
Objective: In obesity, chronic low grade inflammation, created by induction of pro-inflammatory markers, causes adipocyte dysfunction in adipose tissue. Adipocytes dysfunction is associated with various diseases including insulin resistance and obesity. In obesity, inflammatory factors such as osteopontin (OPN), angiopoietin-like protein 2 (Angptl2) and transforming growth factor-β (TGF-β) are...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1985
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)38753-7